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	Provedor de dados ArchiMer (2) Autor Ansquer, Dominique (1) Avarre, Jean-christophe (1) Bonami, Jean-robert (1) Le Moullac, Gilles (1) Levy, Peva (1) Lightner, Donald (1) Mari, Jocelyne (1) Poulos, Bonnie (1) Saulnier, Denis (1) Vonau, Vincent (1) Mais... Palavra-chave Shrimp disease (2) Genomic characterization (1) PCR diagnosis (1) Penaeidae (1) Penaeus (Litopenaeus) stylirostris (1) Small subunit rDNA gene (1) Syndrome 93 (1) Taura syndrome (1) Vibrio penaeicida (1) Vibriosis (1) Virus (1) Mais... Tipo do documento Text (2) Ano 2002 (1) 2000 (1) País France (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia ArchiMer Saulnier, Denis; Avarre, Jean-christophe; Le Moullac, Gilles; Ansquer, Dominique; Levy, Peva; Vonau, Vincent. Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V.... Tipo: Text Palavras-chave: Small subunit rDNA gene; Penaeus (Litopenaeus) stylirostris; Vibriosis; Shrimp disease; PCR diagnosis; Vibrio penaeicida; Syndrome 93. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-4436.pdf Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses ArchiMer Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert. The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of... Tipo: Text Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae. Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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